HPLC Column, RP C4 Butyl, Wide Pore, 3um, 4.6mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of proteins, peptides, enzymes, and other large biomolecules. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger molecules compared to standard 100 Å pore columns. The wide pore structure improves mass transfer and interaction with the stationary phase, making it an excellent choice for bioanalytical, biopharmaceutical, and biomolecule characterization applications.
68304-15P-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 150 mm format. The 150 mm length provides maximum resolution and peak capacity for complex biomolecule separations, while the 3 µm particles deliver greater efficiency and sharper peaks than comparable 5 µm packings. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and instrument configuration.
This column is particularly well suited for proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules analyzed by reversed-phase HPLC. The 300 Å pore structure allows large molecules to fully access the bonded phase, improving recovery and separation performance. The C4 chemistry provides lower hydrophobic retention than C8 phases, which is often advantageous for biomolecules that may be excessively retained on more hydrophobic stationary phases. The 150 mm length maximizes resolving power for demanding biopharmaceutical and bioanalytical applications.
Key Benefits
RP C4 Butyl™ Wide Pore stationary phase
Fully double end capped
Traditional butyl (C4) bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for proteins and peptides
3 µm particle technology
Greater efficiency than 5 µm columns
Maximum peak capacity
Excellent for bioanalytical methods
Typical Applications
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
Biopharmaceutical analysis
Protein purity testing
Peptide mapping
Biomolecule separations
Method development
Complex sample characterization
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl™ Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 4.6 mm ID x 150 mm
Length: 150 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve access to the bonded phase for larger molecules
Suitable for conventional HPLC systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 150 mm length maximizes resolution and peak capacity for complex biomolecule separations
Lower retention than comparable C8 stationary phases
Excellent choice for biopharmaceutical and biotechnology laboratories
Particularly useful for large hydrophobic and neutral biomolecules